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| 1. |  | NARDIN, E. C. de; LIEBSCH, D.; MIKICH, S. B. Riqueza e abundância de espécies zoocóricas como preditores do uso de habitats por macacos-prego (Sapajus nigritus) em mosaicos florestais. In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 12., 2013, Colombo. Anais. Colombo: Embrapa Florestas, 2013. (Embrapa Florestas. Documentos, 253). Editores técnicos: Marcílio José Thomazini, Elenice Fritzsons, Patrícia Raquel Silva, Guilherme Schnell e Schuhli, Denise Jeton Cardoso, Luziane Franciscon. EVINCI. Resumos.| Biblioteca(s): Embrapa Florestas. |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
05/08/2016 |
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Data da última atualização: |
16/05/2017 |
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Autoria: |
RIVAS, E. B.; BÔDI, E. C. de A.; HARAKAVA, R.; GREGORI, F.; GONÇALVES, M. C. |
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Afiliação: |
ELIANA BORGES RIVAS, INSTITUTO BIOLÓGICO; ESTEVÃO CARDOSO DE ALMEIDA BÔDI; RICARDO HARAKAVA, IB/CPDSV; FÁBIO GREGORI, FMVZ/USP; MARCOS CESAR GONÇALVES, IB/CPDSV. |
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Título: |
Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 51, n. 5, p. 615-622, maio 2016. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Ocorrência e análise molecular de vírus quarentenário em áreas de cultivo de lírios no Brasil. |
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Conteúdo: |
The objective of this work was to describe the occurrence of quarantine Tulip breaking virus (TBV, synonym Lily mottle virus ? LMoV) and Lily symptomless virus (LSV), and their respective molecular analyses, to provide data for supporting TBV removal from the Brazilian A1 quarantine pest list, since this virus has spread among the main commercial lily crops in Brazil. The occurrence of these viruses was detected in 12 cultivation areas through multiplex reverse transcription (RT?PCR), using specific primers to genes encoding the respective coat proteins (CP). Eight fragments of 800 nucleotides (nt) obtained from the LMoV?infected lilies and nine fragments of 600 nt from LSV?infected lilies were sequenced. Phylogenetic tree reconstruction showed a robust branch containing the LMoV Brazilian sequences, other LMoV isolates, TBV, and Tulip band breaking virus, suggesting that all are LMoV isolates, although they are clustered into two subgroups. Phylogenetic analysis also showed a robust branch supporting all Brazilian and other LSV sequences, except for an LSV Japanese isolate. Recombination analyses also showed an LMoV recombinant isolate, whereas no recombination events were found among LSV isolates. Lily mottle virus is the prevalent virus in lily crops in Brazil, in single and mixed infections with LSV or Cucumber mosaic virus (CMV). |
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Palavras-Chave: |
Análise de recombinação; Recombination analysis; RT-PCR. |
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Thesagro: |
Filogenia. |
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Thesaurus NAL: |
Lilium; Lily mottle virus; Lily symptomless virus; Phylogeny; Reverse transcriptase polymerase chain reaction. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/146133/1/Occurrence-and-molecular.pdf
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Marc: |
LEADER 02337naa a2200289 a 4500
001 2050313
005 2017-05-16
008 2016 bl uuuu u00u1 u #d
100 1 $aRIVAS, E. B.
245 $aOccurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil.
260 $c2016
500 $aTítulo em português: Ocorrência e análise molecular de vírus quarentenário em áreas de cultivo de lírios no Brasil.
520 $aThe objective of this work was to describe the occurrence of quarantine Tulip breaking virus (TBV, synonym Lily mottle virus ? LMoV) and Lily symptomless virus (LSV), and their respective molecular analyses, to provide data for supporting TBV removal from the Brazilian A1 quarantine pest list, since this virus has spread among the main commercial lily crops in Brazil. The occurrence of these viruses was detected in 12 cultivation areas through multiplex reverse transcription (RT?PCR), using specific primers to genes encoding the respective coat proteins (CP). Eight fragments of 800 nucleotides (nt) obtained from the LMoV?infected lilies and nine fragments of 600 nt from LSV?infected lilies were sequenced. Phylogenetic tree reconstruction showed a robust branch containing the LMoV Brazilian sequences, other LMoV isolates, TBV, and Tulip band breaking virus, suggesting that all are LMoV isolates, although they are clustered into two subgroups. Phylogenetic analysis also showed a robust branch supporting all Brazilian and other LSV sequences, except for an LSV Japanese isolate. Recombination analyses also showed an LMoV recombinant isolate, whereas no recombination events were found among LSV isolates. Lily mottle virus is the prevalent virus in lily crops in Brazil, in single and mixed infections with LSV or Cucumber mosaic virus (CMV).
650 $aLilium
650 $aLily mottle virus
650 $aLily symptomless virus
650 $aPhylogeny
650 $aReverse transcriptase polymerase chain reaction
650 $aFilogenia
653 $aAnálise de recombinação
653 $aRecombination analysis
653 $aRT-PCR
700 1 $aBÔDI, E. C. de A.
700 1 $aHARAKAVA, R.
700 1 $aGREGORI, F.
700 1 $aGONÇALVES, M. C.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 51, n. 5, p. 615-622, maio 2016.
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Embrapa Unidades Centrais (AI-SEDE) |
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